| RSPO2

R-spondin 2 also known as roof plate-specific spondin-2 is a secreted protein that that is encoded by the RSPO2 gene.R-spondin 2 synergizes with canonical WNT to activate beta-catenin. RSPO2 has been proposed to regulate craniofacial patterning and morphogenesis within pharyngeal arch 1 through ectoderm-mesenchyme signaling via the endothelin-Dlx5/6 pathway.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312	156	78	0
OD	2.68	1.74	1.01	0.67	0.41	0.28	0.2	0.09
Corrected OD	2.59	1.65	0.92	0.58	0.32	0.19	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse RSPO2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	101

 

| Linearity

Samples were spiked with high concentrations of Mouse RSPO2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	85-95	88-96	90-110
	Average (%)	91	93	95
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	110